期刊
ACS APPLIED NANO MATERIALS
卷 5, 期 3, 页码 4018-4027出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsanm.2c00011
关键词
superparamagnetic iron oxide nanomaterial; magnetic saturation; single-particle blinking; localization-based microscopy; multimodal imaging
资金
- Council of Scientific and Industrial Research [CSIR SRF:09/1058(0014)/2019-EMR-I]
- SERB core research grant (CRG) India [CRG/2020/000268]
Researchers have designed fluorescent SPIONs with a size of 16 nm, which exhibit significant magnetic saturation and high single-particle photon counts for optical-based super-resolution microscopy. These SPIONs can be used to stain and image lysosomes with high contrast, achieving super-resolution imaging down to 130 nm.
Superparamagnetic iron oxide nanoparticles (SPIONs) have become benchmark materials for their large magnetic saturation and excellent T-2 contrast. Despite the large magnetic saturation that arises due to the enormous iron content, incorporating a fluorescent nature for their use as multimodal probes drastically reduces the magnetic saturation. To achieve the desired magnetic saturation in fluorescence-decorated SPIONs, the size is increased substantially to hundreds of nanometers. Here, we rationally design fluorescent SPIONs of size similar to 16 nm with significant magnetic saturation and high single-particle photon counts used for optical-based super-resolution microscopy. The SPIONs were highly specific to stain and image the lysosomes of HeLa cells with high contrast and were capable of providing super-resolution radial fluctuation (SRRF) imaging of lysosomes down to 130 nm, which is much lower than the diffraction limit in light microscopy.
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