期刊
JOURNAL OF CELL SCIENCE
卷 129, 期 16, 页码 3077-3083出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.191262
关键词
Caveolae; Caveolar coat; Caveolin; Cavin; Cryo-electron tomography
类别
资金
- Ministry of Education - Singapore AcRF TIER1 grant [RG39/14]
- Medical Research Council [MC_U105178778]
- MRC [MC_U105178778] Funding Source: UKRI
- Medical Research Council [MC_U105178778] Funding Source: researchfish
Caveolae are specialized membrane domains that are crucial for the correct function of endothelial cells, adipocytes and muscle cells. Caveolins and cavins are both required for caveolae formation, and assemble into a large (80S) caveolar coat complex (80S-CCC). The architecture of the 80S-CCC, however, has not been analyzed. Here, we study the 80S-CCC isolated from mammalian cells using negative stain electron microscopy and 3D cryo-electron tomography. We show that the 80S-CCC is a hollow sphere with a diameter of 50-80 nm, and so has the same size and shape as individual caveolar bulbs. This provides strong evidence that the distinctive membrane shape of caveolae is generated by the shape of the 80S-CCC itself. The particle appears to be made up of two layers, an inner coat composed of polygonal units of caveolins that form a polyhedral cage, and an outer filamentous coat composed of cavins. The data suggest that the peripheral cavin coat is aligned along the edges of the inner polyhedral cage, thereby providing a mechanism for the generation of a morphologically stable caveolar coat.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据