期刊
LIFE SCIENCE ALLIANCE
卷 5, 期 6, 页码 -出版社
LIFE SCIENCE ALLIANCE LLC
DOI: 10.26508/lsa.202101226
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- Swiss National Science Foundation [PP00P3_157486, PP00P3_183721, PP00P3_190073]
- Swiss National Science Foundation (SNF) [PP00P3_157486, PP00P3_190073, PP00P3_183721] Funding Source: Swiss National Science Foundation (SNF)
The protein RNH1 has been found to inhibit the activation of inflammasomes, reducing inflammation. Deletion of RNH1 in macrophages leads to increased production of IL-1β and activation of caspase-1. Furthermore, RNH1 protein levels were negatively correlated with disease severity and inflammation in COVID-19 patients.
Inflammasomes are cytosolic innate immune sensors of pathogen infection and cellular damage that induce caspase-1-mediated inflammation upon activation. Although inflammation is protective, uncontrolled excessive inflammation can cause inflammatory diseases and can be detrimental, such as in coronavirus disease (COVID-19). However, the underlying mechanisms that control inflammasome activation are incompletely understood. Here we report that the leucinehomology with LRRs of NLRP (nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain containing) proteins, attenuates inflammasome activation. Deletion of RNH1 in macrophages increases interleukin (IL)-1 beta production and caspase-1 activation in response to inflammasome stimulation. Mechanistically, RNH1 decreases pro-IL-1 beta expression and induces proteasome-mediated caspase-1 degradation. Corroborating this, mouse models of monosodium urate (MSU)-induced peritonitis and lipopolysaccharide (LPS)induced endotoxemia, which are dependent on caspase-1, respectively, show increased neutrophil infiltration and lethality in Rnh1(-/-) mice compared with wild-type mice. Furthermore, RNH1 protein levels were negatively related with disease severity and inflammation in hospitalized COVID-19 patients. We propose that RNH1 is a new inflammasome regulator with relevance to COVID-19 severity.
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