期刊
FRONTIERS IN ENVIRONMENTAL SCIENCE
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fenvs.2022.825112
关键词
carbon dots; nanomaterial; fluorescent biosensor; pesticide; chlorpyrifos; enzyme
资金
- Ministry of Science, Technology, and Innovation of the Colombian government [111571451059, FP44842-147-2016, 133380764415, CT-800-2018]
In this study, a fluorescent biosensor was developed by conjugating carbon quantum dots with acetylcholinesterase to detect chlorpyrifos in commercial pesticide formulations. The biosensor showed high selectivity and sensitivity, with a limit of detection as low as 0.14 ppb for chlorpyrifos.
Chlorpyrifos formulations are the most used in Colombia and other countries for crop protection, and their presence has been proven in many water sources. The application of nanomaterials, including carbon quantum dots (CD), can significantly improve the performance of optical biosensors for quick and accurate detection of these pesticides. In this work, naturally fluorescent and nontoxic CD were conjugated with acetylcholinesterase (AChE) as a bioreceptor, to produce a fluorescent biosensor. This system was modulated with graphene oxide (GO), showing a fluorescence recovery in the presence of the pesticide. The biosensor was evaluated for the detection of pure chlorpyrifos (CPF), profenofos (PF), and a commercial formulation called Lorsban (R). A limit of detection (LOD) as low as 0.14 and 2.05 ppb for chlorpyrifos and Lorsban (R), respectively, was obtained. Profenofos did not show any recovery with this system at the evaluated concentrations. The system also showed a good selectivity in the presence of proteins (BSA) and other organic substances (Glucose) usually present in drinking water. The specificity was also evaluated against cypermethrin and methomyl commercial formulations showing minimal interference. To the best of our knowledge, this is one of the few works reporting the detection of commercial pesticide formulations with promising results.
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