4.6 Article

Bladder Cancer Cells Exert Pleiotropic Effects on Human Adipose-Derived Stem Cells

期刊

LIFE-BASEL
卷 12, 期 4, 页码 -

出版社

MDPI
DOI: 10.3390/life12040549

关键词

bladder cancer; adipose-derived stem cells; cell-based therapies; cancer recurrence

资金

  1. research grant for young scietists (MN-1WL)

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Stem cell-based therapies are considered promising for treating bladder cancer. However, the delivery of cells producing paracrine factors may disrupt the microenvironment and potentially reactivate tumors. In this study, we investigated the interaction between adipose-derived stem cells (ASCs) and bladder cancer cells. We found that ASCs increased MCP-1 secretion by bladder cancer cells and altered their morphology. Additionally, soluble factors secreted by bladder cancer cells decreased ASC numbers and affected their behavior. Our results suggest that the interactions between ASCs and bladder cancer cells have a significant impact on the non-muscle-invasive urinary bladder.
Stem cell-based therapies are considered one of the most promising disciplines in biomedicine. Bladder cancer patients could benefit from therapies directed to promote healing after invasive surgeries or to lessen urinary incontinence, a common side effect of both cancer itself and the treatment. However, the local delivery of cells producing large amounts of paracrine factors may alter interactions within the microenvironment. For this reason, reconstructive cellular therapies for patients with a history of cancer carry a potential risk of tumor reactivation. We used an indirect co-culture model to characterize the interplay between adipose-derived stem cells and bladder cancer cells. Incubation with ASCs increased MCP-1 secretion by bladder cancer cells (from 2.1-fold to 8.1-fold, depending on the cell line). Cancer cell-derived factors altered ASC morphology. Cells with atypical shapes and significantly enlarged volumes appeared within the monolayer. Incubation in a conditioned medium (CM) containing soluble mediators secreted by 5637 and HB-CLS-1 bladder cancer cell lines decreased ASC numbers by 47.5% and 45.7%. A significant increase in adhesion to ECM components, accompanied by reduced motility and sheet migration, was also observed after incubation in CM from 5637 and HB-CLS-1 cells. No differences were observed when ASCs were co-cultured with HT-1376 cells. Our previous and present results indicate that soluble mediators secreted by ASCs and bladder cancer cells induce opposite effects influencing cells that represent the non-muscle-invasive urinary bladder.

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