期刊
LIFE-BASEL
卷 12, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/life12040578
关键词
neurotoxicity; silver nanoparticles; quercetin; gene expression; immunohistochemistry; brain
资金
- Taif University, Saudi Arabia [TURSP-2020/197]
This study investigated the neurotoxicity induced by silver nanoparticles (AgNPs) and the neuroprotective effects of quercetin. The results showed that AgNP exposure increased oxidative stress and inflammation in the brain tissue, while quercetin effectively counteracted the negative effects of AgNPs by modulating tight junction proteins, antioxidant systems, and inflammatory responses.
This study aimed to investigate the oxidative neurotoxicity induced by silver nanoparticles (AgNPs) and assess the neuroprotective effects of quercetin against this toxicity. Forty adult male rats were divided into four equal groups: control, AgNPs (50 mg/kg intraperitoneally), quercetin (50 mg/kg orally), and quercetin + AgNPs. After 30 days, blood and brain tissue samples were collected for further studies. AgNP exposure increased lipid peroxidation and decreased glutathione peroxidase, catalase, and superoxide dismutase activities in brain tissue. AgNPs decreased serum acetylcholine esterase activity and gamma-aminobutyric acid concentrations. AgNPs upregulated tumor necrosis factor-alpha, interleukin-1 beta, and Bax transcript levels. AgNPs reduced the transcripts of claudin-5, brain-derived neurotrophic factor, paraoxonase, nuclear factor-erythroid factor 2 (Nrf2), and Bcl-2. Histopathologically, AgNPs caused various degenerative changes and neuronal necrosis associated with glial cell reactions. AgNPs increased the immunohistochemical staining of glial fibrillary acidic protein (GFAP) in the cerebrum and cerebellum. Oral treatment with quercetin efficiently counteracted the opposing effects of AgNPs on brain tissue via modulation of tight junction proteins, Nrf2, and paraoxonase, and its positive mechanism in modulating pro-inflammatory cytokines and the downregulation of GFAP expression, and the apoptotic pathway. AgNPs also altered the severity of histopathological lesions and modulated GFAP immunostaining in the examined tissue.
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