4.5 Article

A Microphysiometric System Based on LAPS for Real-Time Monitoring of Microbial Metabolism

期刊

CHEMOSENSORS
卷 10, 期 5, 页码 -

出版社

MDPI
DOI: 10.3390/chemosensors10050177

关键词

microphysiometer; LAPS; pH measurement; biosensor; microbial metabolism

资金

  1. National Natural Science Foundation of China [62120106004]
  2. National Key Research and Development Program of China [2021YFF1200803, 2021YFB3200801, SQ2021AAA010032]
  3. Natural Science Foundation of Zhejiang Province [LBY21H180001, LY21C100001]
  4. China Postdoctoral Science Foundation Funded Project [2020M671728, 2021T140605]

向作者/读者索取更多资源

This article introduces a new method for microbial metabolism detection. By using a light addressable potentiometric sensor (LAPS) and a microfluidic LAPS chip, the decrease in pH caused by glucose fermentation in Lactobacillus rhamnosus was successfully measured. This method has the potential to be applied in metabolism detection of microorganisms and even 3D cells and organoids.
Macronutrients (carbohydrates, fat and protein) are the cornerstones of daily diet, among which carbohydrates provide energy for the muscles and central nervous system during movement and exercise. The breakdown of carbohydrates starts in the oral cavity, where they are primarily hydrolyzed to glucose and then metabolized to organic acids. The end products may have an impact on the oral microenvironment, so it is necessary to monitor the process of microbial metabolism and to measure the pH change. Although a pH meter has been widely used, it is limited by its sensitivity. We then introduce a light addressable potentiometric sensor (LAPS), which has been used in extracellular acidification detection of living cells with the advantages of being objective, quantitative and highly sensitive. However, it is difficult to use in monitoring bacterial metabolism because bacteria cannot be immobilization on the LAPS chip as easily as living cells. Therefore, a microphysiometric system integrated with Transwell insert and microfluidic LAPS chip was designed and constructed to solve this problem. The decrease in pH caused by glucose fermentation in Lactobacillus rhamnosus was successfully measured by this device. This proves the feasibility of the system for metabolism detection of non-adhere targets such as microorganisms and even 3D cells and organoids.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据