期刊
BIOSENSORS-BASEL
卷 12, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/bios12050351
关键词
total internal reflection ellipsometry; SARS-CoV-2; immune complex; antibody-antigen interaction
资金
- European Regional Development Fund [13.1.1-LMT-K-718-05-0033]
- Research Council of Lithuania (LMTLT)
This study investigated the interactions between antibodies produced after vaccination and three SARS-CoV-2 variants. The results showed that the antibodies can effectively protect against these variants.
SARS-CoV-2 vaccines provide strong protection against COVID-19. However, the emergence of SARS-CoV-2 variants has raised concerns about the efficacy of vaccines. In this study, we investigated the interactions of specific polyclonal human antibodies (pAb-SCoV2-S) produced after vaccination with the Vaxzevria vaccine with the spike proteins of three SARS-CoV-2 variants of concern: wild-type, B.1.1.7, and B.1.351. Highly sensitive, label-free, and real-time monitoring of these interactions was accomplished using the total internal reflection ellipsometry method. Thermodynamic parameters such as association and dissociation rate constants, the stable immune complex formation rate constant (kr), the equilibrium association and dissociation (K-D) constants and steric factors (P-s) were calculated using a two-step irreversible binding mathematical model. The results obtained show that the K-D values for the specific antibody interactions with all three types of spike protein are in the same nanomolar range. The K-D values for B.1.1.7 and B.1.351 suggest that the antibody produced after vaccination can successfully protect the population from the alpha (B.1.1.7) and beta (B.1.351) SARS-CoV-2 mutations. The steric factors (P-s) obtained for all three types of spike proteins showed a 100-fold lower requirement for the formation of an immune complex when compared with nucleocapsid protein.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据