期刊
BIOLOGY-BASEL
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/biology11050664
关键词
beta-1,4-endoglucanase; endo-polygalacturonase; grape pomace; grape cell wall; genetically modified commercial wine yeast
类别
资金
- Wine industry network of expertise and technology (Winetech) [IWBT P14/03, IWBT P14/04]
- Technology and Human Resources for Industry Programme (THRIP) [TP 13081327560]
- National Research Fund of South Africa (NRF) [92290]
This study demonstrated the utilization of industrial wine yeast strains expressing hydrolytic enzymes in fermenting Chardonnay pomace, unraveling cell walls of berry tissues. Recombinant yeast strains producing endo-polygalacturonase and endo-glucanase are effective in modifying berry tissue cell walls and enriching rhamnogalacturonan-type polymers.
Industrial wine yeast strains expressing hydrolytic enzymes were fermented on Chardonnay pomace and were shown to unravel the cell walls of the berry tissues according to the enzyme activities. The yeasts produced a native endo-polygalacturonase (Saccharomyces cerevisiae x Saccharomyces paradoxus hybrid, named PR7) and/or a recombinant endo-glucanase (S. cerevisiae strains named VIN13 END1 and PR7 END1). The impact of the enzymes during the fermentations was evaluated by directly studying the cell wall changes in the berry tissues using a Comprehensive Microarray Polymer Profiling technique. By the end of the fermentation, the endo-glucanase did not substantially modify the berry tissue cell walls, whereas the endo-polygalacturonase removed some homogalacturonan. The recombinant yeast strain producing both enzymes (PR7 END1) unravelled the cell walls more fully, enabling polymers, such as rhamnogalacturonan-I, beta-1,4-D-galactan and alpha-1,5-L-arabinan, as well as cell wall proteins to be extracted in a pectin solvent. This enzyme synergism led to the enrichment of rhamnogalacturonan-type polymers in the subsequent NaOH fractions. This study illustrated the potential utilisation of a recombinant yeast in pomace valorisation processes and simulated consolidated bioprocessing. Furthermore, the cell wall profiling techniques were confirmed as valuable tools to evaluate and optimise enzyme producing yeasts for grape and plant cell wall degradation.
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