4.7 Article

Structural and biochemical characterization of in vivo assembled Lactococcus lactis CRISPR-Csm complex

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COMMUNICATIONS BIOLOGY
卷 5, 期 1, 页码 -

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NATURE PORTFOLIO
DOI: 10.1038/s42003-022-03187-1

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  1. NIH [S10 RR025080, R01 GM099604, R35GM118160]
  2. [S10 RR024564]
  3. [U24 GM116788]

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This study investigates the structure and activity of the Lactococcus lactis Csm complex in different functional states using cryoEM, functional assays, and comparative cross-linking. The results suggest that RNA binding influences the conformation and protein dynamics of the Csm complex.
The small RNA-mediated immunity in bacteria depends on foreign RNA-activated and self RNA-inhibited enzymatic activities. The multi-subunit Type III-A CRISPR-Cas effector complex (Csm) exemplifies this principle and is in addition regulated by cellular metabolites such as divalent metals and ATP. Recognition of the foreign or cognate target RNA (CTR) triggers its single-stranded deoxyribonuclease (DNase) and cyclic oligoadenylate (cOA) synthesis activities. The same activities remain dormant in the presence of the self or non-cognate target RNA (NTR) that differs from CTR only in its 3 '-protospacer flanking sequence (3 '-PFS). Here we employ electron cryomicroscopy (cryoEM), functional assays, and comparative cross-linking to study in vivo assembled mesophilic Lactococcus lactis Csm (LlCsm) at the three functional states: apo, the CTR- and the NTR-bound. Unlike previously studied Csm complexes, we observed binding of 3 '-PFS to Csm in absence of bound ATP and analyzed the structures of the four RNA cleavage sites. Interestingly, comparative crosslinking results indicate a tightening of the Csm3-Csm4 interface as a result of CTR but not NTR binding, reflecting a possible role of protein dynamics change during activation. This study presents Cryo-EM structures of the Lactococcus lactis Type III-A CRISPR-Cas effector complex in three functional states, including bound forms with cognate and non-cognate target RNA. The results suggest changing protein dynamics during effector complex activation.

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