期刊
COMMUNICATIONS BIOLOGY
卷 5, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s42003-022-03356-2
关键词
-
资金
- Natural Sciences and Engineering Research Council (NSERC) [RGPIN-2018-05838, RGPIN-2019-06378]
The Panx1 channels have both pro- and anti-convulsant effects in a zebrafish model, shedding light on their role in seizure development. RNA-seq analysis showed distinct metabolic and cell signaling states associated with the loss of Panx1a. The activities of both Panx1 channels affect ATP release and involve the P2rx7 receptor.
The Panx1a and Panx1b (pannexin) ATP release channels exhibit pro- and anti-convulsant effects, respectively, in a zebrafish model, providing further insight into the role of pannexins in seizure development. The molecular mechanisms of excitation/inhibition imbalances promoting seizure generation in epilepsy patients are not fully understood. Evidence suggests that Pannexin1 (Panx1), an ATP release channel, modulates the excitability of the brain. In this report, we performed electrophysiological, behavioral, and molecular phenotyping experiments on zebrafish larvae bearing genetic or pharmacological knockouts of Panx1a and Panx1b channels, each homologous to human PANX1. When Panx1a function is lost, or both channels are under pharmacological blockade, seizures with ictal-like events and seizure-like locomotion are reduced in the presence of pentylenetetrazol. Transcriptome profiling by RNA-seq demonstrates a spectrum of distinct metabolic and cell signaling states which correlate with the loss of Panx1a. Furthermore, the pro- and anticonvulsant activities of both Panx1 channels affect ATP release and involve the purinergic receptor P2rx7. Our findings suggest a subfunctionalization of Panx1 enabling dual roles in seizures, providing a unique and comprehensive perspective to understanding seizure mechanisms in the context of this channel.
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