4.6 Article

Heterologous Expression of Secondary Metabolite Genes in Trichoderma reesei for Waste Valorization

期刊

JOURNAL OF FUNGI
卷 8, 期 4, 页码 -

出版社

MDPI
DOI: 10.3390/jof8040355

关键词

heterologous expression; PKS-NRPS; PKS; waste valorization; microbial cell factory; Trichoderma reesei

资金

  1. German Academic Exchange Service (DAAD)
  2. Egyptian Ministry of Higher Education and Scientific Research (MHESR) [57311832]
  3. DFG [CO 1328/2-1, INST 187/621-1, INST 187/686-1]
  4. Open Access Fund of the Leibniz Universitat Hannover

向作者/读者索取更多资源

Trichoderma reesei has been developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. By inactivating sorbicillinoid biosynthesis and constructing vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes, this strain can produce desired natural products on waste materials. This new method provides a way to convert waste biomass into secondary metabolites.
Trichoderma reesei (Hypocrea jecorina) was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, aspks1) from Acremonium strictum and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, tenS + tenC) from Beauveria bassiana. The resulting engineered T. reesei strains were able to produce the expected natural products 3-methylorcinaldehyde and pretenellin A on waste materials including potato, orange, banana and kiwi peels and barley straw. Developing T. reesei as a heterologous host for secondary metabolite production represents a new method for waste valorization by the direct conversion of waste biomass into secondary metabolites.

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