4.6 Article

Mushrooms Do Produce Flavonoids: Metabolite Profiling and Transcriptome Analysis of Flavonoid Synthesis in the Medicinal Mushroom Sanghuangporus baumii

期刊

JOURNAL OF FUNGI
卷 8, 期 6, 页码 -

出版社

MDPI
DOI: 10.3390/jof8060582

关键词

flavonoids; mushroom; metabolic engineering; Sanghuangporus baumii; phenylalanine ammonia lyase

资金

  1. National Natural Science Foundation of China [32171792]

向作者/读者索取更多资源

This study explores the synthesis of flavonoids in the medicinal mushroom Sanghuangporus baumii through flavonoid-targeted metabolomics and transcriptome analysis. The mushroom synthesized multiple classes of flavonoids on a chemically defined medium, but most of the genes involved in flavonoid biosynthesis were missing. The results suggest that the flavonoid synthesis pathway in S. baumii is different from that in known plants and may involve replacement of missing genes by distantly related ones.
Mushrooms produce a large number of medicinal bioactive metabolites with antioxidant, anticancer, antiaging, and other biological activities. However, whether they produce flavonoids and, if so, how they synthesize them remains a matter of some debate. In the present study, we combined flavonoid-targeted metabolomics and transcriptome analysis to explore the flavonoid synthesis in the medicinal mushroom Sanghuangporus baumii. The S. baumii synthesized 81 flavonoids on a chemically defined medium. The multiple classes of flavonoids present were consistent with the biosynthetic routes in plants. However, paradoxically, most of the genes that encode enzymes involved in the flavonoid biosynthetic pathway are missing from S. baumii. Only four genes related to flavonoid synthesis were found in S. baumii, among which phenylalanine ammonia lyase gene (PAL) is a key gene regulating flavonoid synthesis, and overexpression of SbPAL increases the accumulation of flavonoids. These results suggest that the flavonoid synthesis pathway in S. baumii is different from that in known plants, and the missing genes may be replaced by genes from the same superfamilies but are only distantly related. Thus, this study provides a novel method to produce flavonoids by metabolic engineering using mushrooms.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据