期刊
JOURNAL OF FUNGI
卷 8, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/jof8040404
关键词
Mucorales; anaerobiosis; yeast-like growth; dimorphism; transporter; CAZymes
资金
- NKFI project [K131796, ITM NKFIA TKP-2021-EGA-28]
- Premium Postdoctoral Fellowship Program of the Hungarian Academy of Sciences [460050]
- Janos Bolyai Research Scholarship [BO/00522/19/8]
- Hungarian Academy of Sciences
- uNKP-21-5-SZTE-574-New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development, and Innovation Fund
- uNKP-20-4-I New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund
This study reveals significant changes in gene expression of Mucor lusitanicus under aerobic and anaerobic conditions through transcriptomic analysis. Carbohydrate metabolism and transporter genes, especially sugar transporters, are found to be affected. Moreover, transcripts related to amino acid transport and metabolism, lipid transport and metabolism, and energy production and conversion are downregulated under anaerobic conditions.
Mucor lusitanicus and some other members of the fungal order Mucorales display the phenomenon of morphological dimorphism. This means that these fungi aerobically produce filamentous hyphae, developing a coenocytic mycelium, but they grow in a multipolar yeast-like form under anaerobiosis. Revealing the molecular mechanism of the reversible yeast-hyphal transition can be interesting for both the biotechnological application and in the understanding of the pathomechanism of mucormycosis. In the present study, transcriptomic analyses were carried out after cultivating the fungus either aerobically or anaerobically revealing significant changes in gene expression under the two conditions. In total, 539 differentially expressed genes (FDR < 0.05, |log(2)FC| >= 3) were identified, including 190 upregulated and 349 downregulated transcripts. Within the metabolism-related genes, carbohydrate metabolism was proven to be especially affected. Anaerobiosis also affected the transcription of transporters: among the 14 up- and 42 downregulated transporters, several putative sugar transporters were detected. Moreover, a considerable number of transcripts related to amino acid transport and metabolism, lipid transport and metabolism, and energy production and conversion were proven to be downregulated when the culture had been transferred into an anaerobic atmosphere.
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