期刊
JOURNAL OF FUNGI
卷 8, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/jof8040329
关键词
edible mushrooms; macrophages; cytokines; PBMCs
资金
- EU
- Greek national funds, through the Operational Program Competitiveness, Entrepreneurship and Innovation under the call RESEARCH-CREATE-INNOVATE [T1EDK-03404]
Recent studies have shown the important role of edible mushrooms and fungal compounds, especially polysaccharides, in human health and disease. This research focuses on the immunomodulatory effects of mushroom polysaccharides, particularly beta-glucans, and their potential prebiotic activity on gut micro-organisms, as well as their link to anticancer properties. This study investigates the immunomodulating effects of Pleurotus eryngii mushrooms, known for their high beta-glucan content, lactogenic effect, and geno-protective properties, on human macrophages and peripheral blood mononuclear cells (PBMCs) using in vitro fermentation.
Recent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially beta-glucans, and the link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-organisms has been the subject of intense research over the last decade. We investigated the immunomodulating effects of Pleurotus eryngii mushrooms, selected due to their high beta-glucan content, strong lactogenic effect, and potent geno-protective properties, following in vitro fermentation by fecal inocula from healthy elderly volunteers (>60 years old). The immunomodulating properties of the fermentation supernatants (FSs) were initially investigated in U937-derived human macrophages. Gene expression as well as pro- (TNF-alpha, IL-1 beta) and anti-inflammatory cytokines (IL-10, IL-1R alpha) were assessed and correlated with the fermentation process. The presence of P. eryngii in the fermentation process led to modifications in immune response, as indicated by the altered gene expression and levels of the cytokines examined, a finding consistent for all volunteers. The FSs immunomodulating effect on the volunteers' peripheral blood mononuclear cells (PBMCs) was verified through the use of cytometry by time of flight (CyTOF) analysis.
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