期刊
BIOMEDICINES
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines10030589
关键词
gap junction; inner-ear development; Cx26; Cx32; Cx37; Cx40; Cx43; Cx45; Panx1; yotari
资金
- Croatian Science Foundation [IP-06-2016-2575]
By investigating the deficiency of DAB1-protein in the inner-ear development of yotari, we found that most connexins were significantly decreased in yotari, compared to wild-type mice and humans. Additionally, the expression of pannexin PANX1 was also significantly lower in yotari, except at certain developmental stages. These results emphasize the importance of connexins in the development of vestibular and cochlear functions and provide potential therapeutic targets for inner-ear impairments.
We investigated DAB1-protein deficiency in the inner-ear development of yotari in comparison to humans and wild-type (wt) mice by immunofluorescence for the expression of connexins (Cxs) and the pannexin Panx1. The spatial and temporal dynamics of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 were determined in the sixth and eighth weeks of human development and at the corresponding mouse embryonic E13.5 and E15.5, in order to examine gap junction intercellular communication (GJIC) and hemichannel formation. The quantification of the area percentage covered by positive signal was performed for the epithelium and mesenchyme of the cochlear and semicircular ducts and is expressed as the mean +/- SD. The data were analysed by one-way ANOVA. Almost all of the examined Cxs were significantly decreased in the cochlear and semicircular ducts of yotari compared to wt and humans, except for Cx32, which was significantly higher in yotari. Cx40 dominated in human inner-ear development, while yotari and wt had decreased expression. The Panx1 expression in yotari was significantly lower than that in the wt and human inner ear, except at E13.5 in the mesenchyme of the wt and epithelium and mesenchyme of humans. Our results emphasize the relevance of GJIC during the development of vestibular and cochlear functions, where they can serve as potential therapeutic targets in inner-ear impairments.
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