期刊
BIOMEDICINES
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines10051063
关键词
mesenchymal stem cells; MSC secretome; MSC extracellular vesicles; Lyosecretome; 3D coprinting; poly(epsilon-caprolactone); alginate hydrogel; bone tissue engineering; bone regenerative medicine
资金
- Interreg V-A Italy-Switzerland 2014-2020-ATEx-Advanced Therapies Experiences [637541]
The study demonstrated that combined printing of Lyosecretome in scaffolds can enhance osteogenic effects, promote MSC differentiation towards bone cells in osteogenic medium, and achieve better bone formation at 14 and 28 days.
Recently, 3D-printed scaffolds for the controlled release of mesenchymal stem cell (MSC) freeze-dried secretome (Lyosecretome) have been proposed to enhance scaffold osteoinduction and osteoconduction; coprinting of poly(epsilon-caprolactone) (PCL) with alginate hydrogels allows adequate mechanical strength to be combined with the modulable kinetics of the active principle release. This study represents the feasibility study for the sterile production of coprinted scaffolds and the proof of concept for their in vitro biological efficacy. Sterile scaffolds were obtained, and Lyosecretome enhanced their colonization by MSCs, sustaining differentiation towards the bone line in an osteogenic medium. Indeed, after 14 days, the amount of mineralized matrix detected by alizarin red was significantly higher for the Lyosecretome scaffolds. The amount of osteocalcin, a specific bone matrix protein, was significantly higher at all the times considered (14 and 28 days) for the Lyosecretome scaffolds. Confocal microscopy further confirmed such results, demonstrating improved osteogenesis with the Lyosecretome scaffolds after 14 and 28 days. Overall, these results prove the role of MSC secretome, coprinted in PCL/alginate scaffolds, in inducing bone regeneration; sterile scaffolds containing MSC secretome are now available for in vivo pre-clinical tests of bone regeneration.
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