期刊
BIOACTIVE MATERIALS
卷 12, 期 -, 页码 120-132出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2021.10.016
关键词
3D printed porous scaffold; Bioactive ceramics; HAp surface layer; Cell fate mediation; Bone augmentation
资金
- National key research and development plan [2017YFC1105000, 2017YFA0205600]
- Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory [2018GZR110102001]
- Science and Technology Program of Guangdong Province [2019B010941002]
- Science and Technology Program of Guangzhou [201804020060, 202007020002]
- Natural Science Foundation of Guangdong Province [2021A1515011741, 2020A1515011354]
- National Nature Science Foundation of China [U1801252]
- National Natural Science Foundation of China [31700823]
- Guangzhou Science and Technology Planning Project [202102020005]
This study utilized a facile and novel method to construct a uniform hydroxyapatite (HAp) surface layer on composite ceramics, and combined with 3D printing technology to fabricate biomimetic hierarchical structure scaffolds. The obtained HAp surface layer favored cell adhesion, upregulated cellular differentiation and gene expression, and promoted capillary formation, bone augmentation, and new bone matrix formation in vivo.
Calcium phosphate bio-ceramics are osteo-conductive, but it remains a challenge to promote the induction of bone augmentation and capillary formation. The surface micro/nano-topography of materials can be recognized by cells and then the cell fate are mediated. Traditional regulation methods of carving surface structures on bioceramics employ mineral reagents and organic additives, which might introduce impurity phases and affect the biological results. In a previous study, a facile and novel method was utilized with ultrapure water as the unique reagent for hydrothermal treatment, and a uniform hydroxyapatite (HAp) surface layer was constructed on composite ceramics (beta-TCP/CaSiO3) in situ. Further combined with 3D printing technology, biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramic scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface layer. The obtained HAp surface layer favoured cell adhesion, alleviated the cytotoxicity of precursor scaffolds, and upregulated the cellular differentiation of mBMSCs and gene expression of HUVECs in vitro. In vivo studies showed that capillary formation, bone augmentation and new bone matrix formation were upregulated after the HAp surface layer was obtained, and the results confirmed that the fabricated biomimetic hierarchical structure scaffold could be an effective candidate for bone regeneration.
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