期刊
FRONTIERS IN VETERINARY SCIENCE
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fvets.2022.844058
关键词
African swine fever (ASF); antiviral; chlorine dioxide; viral attachment; cytokine
资金
- National Natural Science Foundation of China [32072695, 31872329]
- Natural Science Foundation of Guangdong province [2019B1515210024, 2019B020211003]
- Guangdong Provincial Department of Science and Technology
African swine fever (ASF) is a highly contagious disease with no effective vaccine or treatment. This study found that chlorine dioxide (ClO2) can strongly inhibit ASF virus replication and has anti-inflammatory properties. These findings are significant for the development of new drugs against ASF.
African swine fever (ASF) is a highly contagious disease and provokes severe economic losses and health threats. At present no effective vaccine or treatment is available to prevent or cure ASF. Consequently, there is an urgent need to develop effective drugs against ASF virus (ASFV). Chlorine dioxide (ClO2), an ideal biocide, has broad-spectrum antibacterial activity and no drug resistance. Here, we found that ClO2 strongly inhibited ASFV replication in porcine alveolar macrophages (PAMs). The inhibitory effect of ClO2 occurred during viral attachment rather than entry, indicating that ClO2 suppressed the early stage of virus life cycle. ClO2 showed a potent anti-ASFV effect when added either before, simultaneously with, or after virus infection. Furthermore, ClO2 could destroy viral nucleic acids and proteins, which may contribute to its capacity of inactivating ASFV virions. The minimum concentration of degradation of ASFV nucleic acids by ClO2 is 1.2 mu g/mL, and the degradation is a temperature-dependent manner. These have guiding significance for ClO2 prevention and control of ASFV infection in pig farms. In addition, ClO2 decreased the expression of ASFV-induced inflammatory cytokines. Overall, our findings suggest that ClO2 may be an ideal candidate for the development of novel anti-ASFV prophylactic and therapeutic drugs in swine industry.
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