4.7 Article

Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes

期刊

FRONTIERS IN VETERINARY SCIENCE
卷 9, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fvets.2022.832255

关键词

African swine fever virus; recombinant; pseudorabies virus; Red/ET recombineering technology; vaccine

资金

  1. Key Research and Development Program of Guangdong Province [2020B020222001]
  2. National Key Research and Development Program [2021YFD1801205]
  3. Key-Area Research and Development Program of Guangdong Province [2019B020218004]
  4. Guangdong Basic and Applied Basic Research Foundation [2019A1515012006]
  5. Construction of Modern Agricultural Science and Technology Innovation Alliance in Guangdong Province [2020KJ128]

向作者/读者索取更多资源

A recombinant virus expressing African swine fever virus (ASFV) antigen genes was successfully constructed using pseudorabies virus as the viral vector, and its immunogenicity in inducing high levels of antibodies in vivo was validated. These findings are of significance for the development of new ASFV vaccines.
African swine fever (ASF) is a highly contact infectious disease caused by the African swine fever virus (ASFV). The extremely complex structure and infection mechanism make it difficult to control the spread of ASFV and develop the vaccine. The ASFV genome is huge with many antigenic genes. Among them, CP204L (p30), CP530R (pp62), E183L (p54), B646L (p72), and EP402R (CD2v) are involved in the process of the virus cycle, with strong immunogenicity and the ability to induce the body to produce neutralizing antibodies. In this study, the recombinant virus rBartha-K61-pASFV that expresses the above ASFV antigen genes was constructed by Red/ET recombineering technology using pseudorabies virus (PRV) vaccine strain Bartha-K61. Western blot analysis showed that the ASFV antigen gene was expressed and the recombinant virus showed good genetic stability and proliferation characteristics in 15 continuous generations on porcine kidney (PK15) cells. The results of immunoassay of piglets and mice showed that rBartha-K61-pASFV had good immunogenicity and could induce higher antibody levels in the body. Therefore, PRV was a promising viral vector for expressing the ASFV antigen gene, and all the experiments in this study laid a foundation for the further development of a new viral vector vaccine of ASFV.

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