Enhanced Catalytic Activity of a New Nanobiocatalytic System Formed by the Adsorption of Cytochromecon Pluronic Triblock Copolymer Stabilized MoS2Nanosheets

The formation of nanobiohybrids through theimmobilization of enzymes on functional nanomaterials hasopened up exciting research opportunities at the nanobiointerfaces.These systems hold great promise for a wide range of applicationsin biosensing, biocatalytic, and biomedicalfields. Here, we reportthe formation of a hybrid nanobiocatalytic system through theadsorption of cytochromec(Cytc)onpluronictriblockcopolymer, P123 (PEO-b-PPO-b-PEO), stabilized MoS2nano-sheets. The use of pluronic polymer has helped not only to greatlystabilize the exfoliated MoS2nanosheets but also to allow easyadsorption of Cytcon the nanosheets without major structural changes due to its excellent biocompatibility and soft protein-bindingproperty. By comparing the catalytic activity of the Cytc-MoS2nanobiohybrid with that of the free Cytcand as-prepared MoS2nanosheets, we have demonstrated the active role of the nanobiointeractions in enhancing the catalytic activity of the hybrid. Slightstructural perturbation at the active site of the Cytcupon adsorption on MoS2has primarily facilitated the peroxidase activity of theCytc. As the MoS2nanosheets and the native Cytcindividually exhibit weaker intrinsic peroxidase activities, their mutualmodulation at the nanobiointerface has made the Cytc-MoS2a novel nanobiocatalyst with superior activity.

Automated summary

The immobilization of enzymes on functional nanomaterials to form nanobiohybrids offers numerous research opportunities. In this study, a hybrid nanobiocatalytic system was formed through the adsorption of cytochrome c on stabilized MoS2 nanosheets. The nanobiointeractions between the two components enhanced the catalytic activity of the hybrid, making it a novel nanobiocatalyst with superior activity.