Genome Size Variation in Dianthus sylvestris Wulfen sensu lato (Caryophyllaceae)

Genome size (GS) is an important characteristic that may be helpful in delimitation of taxa, and multiple studies have shown correlations between intraspecific GS variation and morphological or environmental factors, as well as its geographical segregation. We estimated a relative GS (RGS) of 707 individuals from 162 populations of Dianthus sylvestris with a geographic focus on the Balkan Peninsula, but also including several populations from the European Alps. Dianthus sylvestris is morphologically variable species thriving in various habitats and six subspecies have been recognized from the Balkan Peninsula. Our RGS data backed-up with chromosome counts revealed that the majority of populations were diploid (2n = 30), but ten tetraploid populations have been recorded in D. sylvestris subsp. sylvestris from Istria (Croatia, Italy). Their monoploid RGS is significantly lower than that of the diploids, indicating genome downsizing. In addition, the tetraploids significantly differ from their diploid counterparts in an array of morphological and environmental characteristics. Within the diploid populations, the RGS is geographically and only partly taxonomically correlated, with the highest RGS inferred in the southern Balkan Peninsula and the Alps. We demonstrate greater RGS variation among the Balkan populations compared to the Alps, which is likely a result of more pronounced evolutionary differentiation within the Balkan Peninsula. In addition, a deep RGS divergence within the Alps likely points to persistence of the alpine populations in different Pleistocene refugia.

Automated summary

The study on genome size variation in Dianthus sylvestris populations from the Balkan Peninsula and the European Alps revealed significant differences between diploid and tetraploid populations, with tetraploids showing genome downsizing and distinct morphological and environmental characteristics compared to diploids. There is geographical and partial taxonomical correlation in relative genome size within diploid populations, with higher RGS in the southern Balkan Peninsula and the Alps. Greater RGS variation was observed among Balkan populations compared to the Alps, indicating more pronounced evolutionary differentiation in the Balkan Peninsula, while deep RGS divergence in the Alps suggests persistence of alpine populations in different Pleistocene refugia.