In Dormant Red Rice Seeds, the Inhibition of Early Seedling Growth, but Not of Germination, Requires Extracellular ABA

The phytohormone abscisic acid (ABA) inhibits seed germination and seedling growth and is required for the inception of dormancy. Xanthoxal (also known as xanthoxin) is the first specific biosynthetic precursor of ABA. In this study, a modified method to produce xanthoxal is described. I tested the ability of either xanthoxal or ABA to reinstate dormancy in dormant red rice seeds whose dormancy was broken by fluridone (an inhibitor of the synthesis of carotenoids and, subsequently, ABA). Xanthoxal was shown to have a stronger inhibitory effect on germination than ABA when exogenously provided. Although this could indicate an additional effect of xanthoxal above that expected if xanthoxal were simply converted to ABA in the seed, alternative hypotheses cannot be excluded. One alternative is that exogenous xanthoxal may be trapped inside the cells to a greater extent than exogenous ABA, resulting in an intracellular level of ABA higher than that reached with a direct application of ABA. As a further alternative, exogenous xanthoxal may interfere with ABA action in the apoplast. In this study, following germination, early seedling growth was delayed only if ABA was applied. This suggests that inhibition of early seedling growth, but not of germination, requires extracellular ABA.

Automated summary

This study describes a modified method to produce xanthoxal, a specific biosynthetic precursor of ABA. Experiments on red rice seeds showed that xanthoxal has a stronger inhibitory effect on germination than ABA. Possible explanations include higher intracellular level of xanthoxal compared to ABA or interference of xanthoxal with extracellular ABA action. Furthermore, only application of ABA delays early seedling growth.