期刊
ENVIRONMENTAL TECHNOLOGY & INNOVATION
卷 26, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.eti.2022.102298
关键词
Bioprocesses; Lignocellulosic biomass; Fungal fermentation; Enzyme statistical design production; Sustainability; Circular economy
资金
- Ministerio de Ciencia, Tecnologia e Innovacion Productiva de Santa Fe [IO2018009]
- Consejo Nacional de Invetigaciones Cientificas y Tecnicas (CONICET) [PUE BD20160041CO]
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT-II-B-2018 2054]
- National University of Rosario [80020180100033UR, 80020180100036UR]
The study optimized the conditions for producing xylanolytic enzymes by Aspergillus niger and Thermomyces lanuginosus on Spartina argentinensis, resulting in an enzymatic extract with higher xylanase specific activity, which holds potential for industrial applications.
Hemicellulose is a major component of plant cell walls and xylan is the most predominant polysaccharide. Xylan degrading enzymes integrate the xylanolytic system. Xylanolytic enzymes were produced by fermentation by Aspergillus niger and Thermomyces lanuginosus grown on grass considered unsuitable for farming called Spartina argentinensis. Significant parameters: type of fermentation used (SmF or SSF), type of leaves of S. argentinensis (green or senescent) and conidia final concentration for xylanase production were screened and optimized. The main results showed that the highest levels of xylanolytic enzyme production were obtained by 1 x 10(5) conidia/mL of A. niger in SmF at 96 h, 30 degrees C, with a mixture of 20.20% of senescent and 79.80% of green leaves. The xylanase specific activity obtained was 62 U/mg, higher than the activity obtained (23 U/mg) in previous work (Taddia et al, 2019), and the concentration of xylanolytic production over that of glucanase activity was maximized five times. The optimized enzymatic extract obtained was characterized by LC-MS and HPLC of carbohydrates. Six enzymes were identified as constituents of the xylanolytic complex and seven carbohydrates. Moreover, the xylanolytic enzyme extract was stable for 30 days at 20 degrees C. Thus, S. argentinensis can be used within the framework of a circular economy, rendering a synergistic combination of the xylanolytic enzymes with industrial applications. (C) 2022 The Author(s). Published by Elsevier B.V.
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