期刊
JOURNAL OF BONE ONCOLOGY
卷 33, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jbo.2022.100415
关键词
Baicalin; Osteosarcoma; Autophagy; Apoptosis; ROS; PI3K gamma
类别
资金
- Natural Science Foundation of Guangdong [2020A1515010003]
- Peaking Planfor the reconstruction of the high-level hospital at Affiliated Hospital of Guangdong Medical University [20501DFY20190168]
- Special Fund for Science and Technology Development of Zhanjiang [2017A01033]
- Young Innovative Talents Project of Guangdong Higher Education Institution [2021KQNCX023]
- Science Fund of Guangdong Medical University [GDMUM201809]
Baicalin exhibits antitumor effects on osteosarcoma cells by reducing proliferation and invasive potential, inducing apoptosis and autophagy, and inhibiting signaling pathways.
Baicalin, a flavonoid derivative, exerts antitumor activity in a variety of neoplasms. However, whether baicalin exerts antitumor effects on osteosarcoma cells remains to be elucidated. In this study, treatment with baicalin reduced the proliferation and invasive potential of osteosarcoma cells and reduced the mitochondrial membrane potential, which eventually caused mitochondrial apoptosis. In addition, baicalin increased intercellular Ca2+ and ROS concentrations. Baicalin-induced apoptosis was confirmed by enhanced Bax, cleaved caspase-3, and cleaved PARP levels and decreased Bcl-2 levels. The increase in LC3-II and p62 suggested that baicalin induced autophagosome formation but ultimately inhibited downstream autophagy. Moreover, apoptosis induced by baicalin was attenuated by the addition of 3-MA. Furthermore, we found that baicalin inhibited the PI3K/Akt/mTOR, ERK1/2 and beta-catenin signaling pathways. Chelation of free Ca2+ by BAPTA-AM also inhibited both apoptosis induction and ROS concentration changes. Finally, NAC pretreatment reversed baicalin treatment outcomes, including the increase in Ca2+ concentration, induction of apoptosis and autophagy, and inhibition of the pathways. Molecular docking results indicated that baicalin might interact with the structural domain of PI3K gamma. Thus, baicalin may be considered a potential candidate for osteosarcoma treatment. (C) 2022 The Authors. Published by Elsevier GmbH.
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