Antimicrobial and Immunomodulatory Effects of Selected Chemokine and Antimicrobial Peptide on Cytokine Profile during Salmonella Typhimurium Infection in Mouse

The antimicrobial and immunomodulatory capacities of the peptide Css54 and the chemokine MCP-1 were tested. The first, a peptide isolated from the venom of the scorpion Centruroides suffusus suffusus was synthesized chemically. In contrast, the second is a monocyte chemoattractant expressed as a recombinant protein in our lab. It was observed in vitro that Css54 inhibited the growth of Salmonella enterica serovar Typhimurium (6.2 mu g/mL). At high concentrations, it was toxic to macrophages (25 mu g/mL), activated macrophage phagocytosis (1.5 mu g/mL), and bound Salmonella LPS (3 mu g/mL). On the other hand, the recombinant MCP-1 neither inhibited the growth of Salmonella Typhimurium nor was it toxic to macrophages (up to 25 mu g/mL), nor activated macrophage phagocytosis or bound Salmonella LPS (up to 3 mu g/mL). Although it was observed in vivo in mice Balb/C that both Css54 and MCP-1 did not resolve the intraperitoneal infection by S. Typhimurium, Css54 decreased the expression of IL-6 and increased IL-10, IL-12p70, and TNF-alpha levels; meanwhile, MCP-1 decreased the expression of IFN-gamma and increased IL-12p70 and TNF-alpha. It was also observed that the combination of both molecules Css54 and MCP-1 increased the expression of IL-10 and TNF-alpha.

Automated summary

The antimicrobial peptide Css54 showed inhibitory effects on the growth of Salmonella enterica serovar Typhimurium, while the recombinant chemokine MCP-1 did not. Css54 was also toxic to macrophages and activated macrophage phagocytosis, while MCP-1 was not. The combination of Css54 and MCP-1 increased the expression levels of IL-10 and TNF-alpha.