4.6 Article

Identification of Metabolomic Biomarkers of Long-Term Stress Using NMR Spectroscopy in a Diving Duck

期刊

METABOLITES
卷 12, 期 4, 页码 -

出版社

MDPI
DOI: 10.3390/metabo12040353

关键词

metabolomics; NMR spectroscopy; lesser scaup; stress physiology; corticosterone; hormone implants; energy metabolism

资金

  1. Environment and Climate Change Canada [419742]
  2. Strategic Applications of Genomics in the Environment
  3. Interprovincial Graduate Student Fellowship
  4. University of Saskatchewan Graduate Scholarship
  5. Wildlife Health Research Fund, WCVM [419146]
  6. Graduate Teaching Fellowship-WCVM

向作者/读者索取更多资源

Human-induced long-term environmental changes have significant impacts on wildlife health. The use of metabolomics in studying stress in birds is still in its early stages. A study on captive lesser scaup ducks found that physiological stress induced by CORT pellets altered energy metabolism and resulted in distinct metabolite profiles compared to the control group.
Human-induced environmental changes that act as long-term stressors pose significant impacts on wildlife health. Energy required for maintenance or other functions may be re-routed towards coping with stressors, ultimately resulting in fluctuations in metabolite levels associated with energy metabolism. While metabolomics approaches are used increasingly to study environmental stressors, its use in studying stress in birds is in its infancy. We implanted captive lesser scaup (Aythya affinis) with either a biodegradable corticosterone (CORT) pellet to mimic the effects of a prolonged stressor or a placebo pellet. 1D H-1 nuclear magnetic resonance (NMR) spectroscopy was performed on serum samples collected over 20 days after implant surgery. We hypothesized that CORT pellet-induced physiological stress would alter energy metabolism and result in distinct metabolite profiles in ducks compared with placebo (control). Quantitative targeted metabolite analysis revealed that metabolites related to energy metabolism: glucose, formate, lactate, glutamine, 3-hydroxybutyrate, ethanolamine, indole-3- acetate, and threonine differentiated ducks with higher circulatory CORT from controls on day 2. These metabolites function as substrates or intermediates in metabolic pathways related to energy production affected by elevated serum CORT. The use of metabolomics shows promise as a novel tool to identify and characterize physiological responses to stressors in wild birds.

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