期刊
METABOLITES
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/metabo12060485
关键词
metabolomics; Gas Chromatographic-Mass Spectrometer(GC-MS); pesticide residue; fermentation broth; flavor substances
资金
- National Natural Science Foundation of China [31601390]
- Key R&D Program of Zhejiang Province [2019C02088]
This study analyzed the changes in flavor substances in wine during fermentation using GC-MS and multivariate statistical methods. The results showed that pesticide residues have a significant impact on the flavor of wine.
The application of pesticides is critical during the growth of high-quality grape for wine making. However, pesticide residues have significant influence on the wine flavor. In this study, gas chromatography-mass spectrometry (GC-MS) was performed and the obtained datasets were analyzed with multivariate statistical methods to investigate changes in flavor substances in wine during fermentation. The principal component analysis (PCA) score plot showed significant differences in the metabolites of wine treated with various pesticides. In trials using five pesticides (hexaconazole, difenoconazole, flutriafol, tebuconazole, and propiconazole), more than 86 metabolites were changed. Most of these metabolites were natural flavor compounds, like carbohydrates, amino acids, and short-chain fatty acids and their derivatives, which essentially define the appearance, aroma, flavor, and taste of the wine. Moreover, the five pesticides added to grape pulp exhibited different effects on the metabolic pathways, involving mainly alanine, aspartate and glutamate metabolism, butanoate metabolism, arginine, and proline metabolism. The results of this study will provide new insight into the potential impact of pesticide residues on the metabolites and sensory profile of wine during fermentation.
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