期刊
PATHOGENS
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/pathogens11030313
关键词
in vitro infectious assays; cell microarrays; immunocytochemistry; SARS-CoV-2; human receptors for viruses; cell cycle and apoptosis
类别
资金
- Portuguese Foundation for Science and Technology (FCT) [109_596696487, 510]
- FCT [SFRH/BD/145217/2019, 2020.04720.BD]
- FEDER-Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020-Operational Program for Competitiveness and Internationalization (POCI), Portugal 2020
- Portuguese funds through FCT/Ministerio da Ciencia, Tecnologia e InovacAo [POCI-01-0145-FEDER-007274]
- Fundação para a Ciência e a Tecnologia [2020.04720.BD, SFRH/BD/145217/2019] Funding Source: FCT
The study proposes a histology-based method for evaluating infection kinetics and demonstrates the advantages of InfectionCMA, which allows for side-by-side comparisons and evaluation of diverse cell lines, infection time points, and biomarker expression.
The recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has forced the scientific community to acquire knowledge in real-time, when total lockdowns and the interruption of flights severely limited access to reagents as the global pandemic became established. This unique reality made researchers aware of the importance of designing efficient in vitro set-ups to evaluate infectious kinetics. Here, we propose a histology-based method to evaluate infection kinetics grounded in cell microarray (CMA) construction, immunocytochemistry and in situ hybridization techniques. We demonstrate that the chip-like organization of the InfectionCMA has several advantages, allowing side-by-side comparisons between diverse cell lines, infection time points, and biomarker expression and cytolocalization evaluation in the same slide. In addition, this methodology has the potential to be easily adapted for drug screening.
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