4.5 Article

Antimicrobial Susceptibility of Microbiota in Bacterial Vaginosis Using Fluorescence In Situ Hybridization

期刊

PATHOGENS
卷 11, 期 4, 页码 -

出版社

MDPI
DOI: 10.3390/pathogens11040456

关键词

antimicrobial resistance; microbiome susceptibility; bacterial vaginosis; dysbiosis; FISH; polymicrobials; metronidazole; octenisept (R); ciclopirox; Lactobacillus crispatus; Saccharomyces boulardii; Gardnerella-phage-endolysin; phagolysin

资金

  1. Charite University research promotion grant [2016-033]
  2. German Federation of Industrial Research Associations ZIM Project [ZF4143701AJ5]

向作者/读者索取更多资源

FISH susceptibility testing allows for a unique evaluation of individually adjusted topical therapy without microbial isolation, facilitating the optimal choice of treatment.
Background: Testing of antibiotic resistance of intact vaginal microbiota in pure culture is not feasible. METHODS: Metronidazole, antiseptic octenisept (R), antimycotic ciclopirox, bacterial probiotic Lactobacillus crispatus, yeast probiotic Saccharomyces boulardii, Gardnerella-phage-endolysin named phagolysin and phagolysin in combination with probiotics were tested for bacteriolytic activity. Included were vaginal swabs from 38 random women with Amsel-confirmed bacterial vaginosis (BV). Test aliquots were incubated by 37 degrees for 2 and 24 h. Gardnerella, low G+C, Atopobium, lactobacilli, Lactobacillus iners and crispatus, Prevotella-Bacteroides, and Gammaproteobacteria microbial groups were quantified using fluorescence in situ hybridization (FISH). Results: The probiotic strain Lactobacillus crispatus demonstrated the weakest bacteriolytical effects, followed by metronidazole. Both had no impact on Gardnerella species, instead lysing Prevotella-Bacteroides, Enterobacteriaceae (by L.crispatus) or LGC, Atopobium and Prevotella-Bacteroides (by metronidazole) groups of the microbiota. Cytolytic activity on Gardnerella was highly pronounced and increased from octenisept to ciclopirox, phagolysin, phagolysin with L.crispatus, being best in the combination of phagolysin with S.boulardii. Universally active ciclopirox and octenisept (R) suppressed nearly all microbial groups including those which are regarded as beneficial. Phagolysin had no effect on naturally occurring Lactobacillus crispatus. Conclusions: FISH susceptibility testing allows unique efficacy evaluation of individually adjusted topical therapy without microbial isolation facilitating optimal therapy choice.

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