期刊
FRONTIERS IN MOLECULAR BIOSCIENCES
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2022.801309
关键词
COVID-19 diagnostics; RT-LAMP; coronavirus; SARS-CoV-2; isothermal amplification; open-source
资金
- Vienna Science and Technology Fund (WWTF) [COV20-031]
- Cambridge Trust LMB Cambridge Scholarship
- Austrian Science Fund [Y 1031-B28, F 80]
- EMBO-YIP
- European Research Council [ERC2015-CoG -682181]
- Boehringer Ingelheim
- Austrian Research Promotion Agency [FFG-852936]
- Austrian Academy of Sciences
- Spanish Ministry of Science and Innovation [PID2019104176RB-I00/AEI/10.13039/501100011033]
- Fundacion Ramon Areces
This article presents a rapid, robust, and sensitive RT-LAMP-based SARS-CoV-2 detection assay that bypasses the RNA isolation step and uses a colorimetric readout. The assay has been optimized and additional steps have been added to increase sensitivity and scalability. Open-source RT-LAMP enzymes and a version for home testing have also been developed.
RT-qPCR-based diagnostic tests play important roles in combating virus-caused pandemics such as Covid-19. However, their dependence on sophisticated equipment and the associated costs often limits their widespread use. Loop-mediated isothermal amplification after reverse transcription (RT-LAMP) is an alternative nucleic acid detection method that overcomes these limitations. Here, we present a rapid, robust, and sensitive RT-LAMP-based SARS-CoV-2 detection assay. Our 40-min procedure bypasses the RNA isolation step, is insensitive to carryover contamination, and uses a colorimetric readout that enables robust SARS-CoV-2 detection from various sample types. Based on this assay, we have increased sensitivity and scalability by adding a nucleic acid enrichment step (Bead-LAMP), developed a version for home testing (HomeDip-LAMP), and identified open-source RT-LAMP enzymes that can be produced in any molecular biology laboratory. On a dedicated website, (DOI: ), we provide detailed protocols and videos. Our optimized, general-purpose RT-LAMP assay is an important step toward population-scale SARS-CoV-2 testing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据