期刊
MICROORGANISMS
卷 10, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms10040678
关键词
Babesia; Theileria; piroplasms; tick-borne disease; cattle; water buffalo
类别
资金
- University of the Philippines Balik
- Japan Society for the Promotion of Science [15H05264, 20KK0154]
- JSPS Bilateral Program [JPJSBP120219936]
- Grants-in-Aid for Scientific Research [15H05264, 20KK0154] Funding Source: KAKEN
Tick-borne protozoan parasites Babesia and Theileria can cause economic losses in the cattle industry. This study investigated the presence of these parasites in large ruminants in the Philippines, confirming the prevalence of T. orientalis and reporting the genetic diversity of B. bovis and T. orientalis.
Babesia and Theileria are tick-borne protozoan parasites that can cause significant economic losses in the cattle industry. This study aimed to contribute to the limited epidemiological data on Theileria orientalis as well as Babesia bigemina and B. bovis in large ruminants in the Philippines. Blood samples of 412 cattle and 108 water buffalo collected from four provinces in Southern Luzon, Philippines, were initially tested for the 18S rRNA gene of piroplasms through nested PCR. Positive samples were further subjected to species-specific PCR. The 18s rRNA of piroplasms was detected in 123 (29.9%) cattle and three (2.8%) water buffaloes. Theileria orientalis was found to be the most common piroplasm in cattle with a detection rate of 17.5%, followed by Babesia bovis and B. bigemina. Co-infections were also observed. Two water buffaloes were found infected with B. bovis, while one was positive for B. bigemina. The phylogenetic tree for B. bovis showed clustering of the isolates in two clades together with isolates from other countries, and a third separate clade. Meanwhile, the T. orientalis isolates in this study were distributed in three clades together with reported isolates from other countries. This study confirms the presence of T. orientalis in the Philippines and reports the genetic diversity of B. bovis and T. orientalis.
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