期刊
MICROORGANISMS
卷 10, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms10040831
关键词
environmental microorganisms; classification; mass spectrometry; MALDI; molecular network
类别
资金
- Investissement d'Avenir grant (CEBA) [ANR-10-LABX-0025]
- CNRS
- CNRS-ICSN
- Region Ile-de-France
In this study, we proposed a workflow using a standardized protein and lipid extraction protocol and reliable spectral comparison algorithm in MetGem software for classification and identification of environmental microorganisms. Our method was validated and shown to be capable of classifying bacteria at the order and genus level, and fungi at the order level.
During the last two decades, MALDI-ToF mass spectrometry has become an efficient and widely-used tool for identifying clinical isolates. However, its use for classification and identification of environmental microorganisms remains limited by the lack of reference spectra in current databases. In addition, the interpretation of the classical dendrogram-based data representation is more difficult when the quantity of taxa or chemotaxa is larger, which implies problems of reproducibility between users. Here, we propose a workflow including a concurrent standardized protein and lipid extraction protocol as well as an analysis methodology using the reliable spectra comparison algorithm available in MetGem software. We first validated our method by comparing protein fingerprints of highly pathogenic bacteria from the Robert Koch Institute (RKI) open database and then implemented protein fingerprints of environmental isolates from French Guiana. We then applied our workflow for the classification of a set of protein and lipid fingerprints from environmental microorganisms and compared our results to classical genetic identifications using 16S and ITS region sequencing for bacteria and fungi, respectively. We demonstrated that our protocol allowed general classification at the order and genus level for bacteria whereas only the Botryosphaeriales order can be finely classified for fungi.
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