期刊
ANTIOXIDANTS
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/antiox11050855
关键词
Arabidopsis thaliana; oxylipin; posttranslational modification; thiol; thioredoxin
资金
- German Science Foundation (Deutsche Forschungsgemeinschaft) [DI 346/22-1, 23-1]
cis-(+)-12-oxophytodienoic acid (OPDA) is a reactive oxylipin that functions as a signaling molecule and regulator by tuning enzyme activities and altering gene expression. This study investigates the reaction of OPDA with specific chloroplast and cytosolic thioredoxins (TRX) and glutaredoxins (GRX) in Arabidopsis thaliana.
cis-(+)-12-Oxophytodienoic acid (OPDA) is a reactive oxylipin produced by catalytic oxygenation of polyunsaturated alpha-linolenic acid (18:3 (omega - 3)) in the chloroplast. Apart from its function as precursor for jasmonic acid synthesis, OPDA serves as a signaling molecule and regulator on its own, namely by tuning enzyme activities and altering expression of OPDA-responsive genes. A possible reaction mechanism is the covalent binding of OPDA to thiols via the addition to the C=C double bond of its alpha,beta-unsaturated carbonyl group in the cyclopentenone ring. The reactivity allows for covalent modification of accessible cysteinyl thiols in proteins. This work investigated the reaction of OPDA with selected chloroplast and cytosolic thioredoxins (TRX) and glutaredoxins (GRX) of Arabidopsis thaliana. OPDA reacted with TRX and GRX as detected by decreased m-PEG maleimide binding, consumption of OPDA, reduced ability for insulin reduction and inability to activate glyceraldehyde-3-phosphate dehydrogenase and regenerate glutathione peroxidase (GPXL8), and with lower efficiency, peroxiredoxin IIB (PRXIIB). OPDAylation of certain protein thiols occurs quickly and efficiently in vitro and is a potent post-translational modification in a stressful environment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据