期刊
ANTIOXIDANTS
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/antiox11030528
关键词
coenzyme Q(10); HPLC with electrochemical detection; electrochemical cells; external quality control; ERNDIM
资金
- Instituto de Salud Carlos III [PI20/00340, FI18/00253]
- European Regional Development Fund A way to make Europe
- FEDER 2014-2020
- Asociacion de Enfermos de Patologias Mitocondriales (AEPMI)
- Asociacion de Enfermos de Patologias Mitocondriales (AEPMI)/Fundacion Ana Carolina Diez Mahou
This article reports a new HPLC-ED method that shows good analytical performance in different biological samples and compares its results with other methods, demonstrating good consistency.
The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra- and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control (EQC) scheme for the plasma CoQ determination between HPLC-ED (Lab 1) and LC-MS/MS (Lab 2) methods shows that the results of the latter were slightly higher in most cases, although a good consistency was generally observed. In conclusion, the new method reported here showed a good analytical performance. The global quality of the EQC scheme results among different participants can be improved with the contribution of more laboratories.
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