期刊
ANTIOXIDANTS
卷 11, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/antiox11061055
关键词
Sargassum; antioxidant; antidiabetic; pancreatic lipase; anti-inflammatory; UHPLC-MS analysis
资金
- Operational Programme for Competitiveness and Internationalization-POCI, within the European Regional Development Fund (FEDER)
- Science and Technology Foundation (FCT)
- [PTDC/BAA-AGR/31015/2017]
- Fundação para a Ciência e a Tecnologia [PTDC/BAA-AGR/31015/2017] Funding Source: FCT
Brown seaweeds, particularly the ethyl acetate fraction, are rich in bioactive compounds called phlorotannins. These compounds have potential antioxidant, inhibitory, and anti-inflammatory properties.
Brown seaweeds are a good source of bioactive compounds, particularly of phlorotannins, which may exert a wide spectrum of pharmacological properties. In the present study, phlorotannins of S. vulgare were extracted using a 70% acetone solution and the crude extract was further purified through liquid-liquid partition, giving rise to n-hexane, ethyl acetate and aqueous residue fractions. The crude extract and the purified fractions were evaluated for potential antioxidant abilities as well as for inhibitory potential towards the digestive enzymes alpha-amylase and pancreatic lipase, and anti-inflammatory potential through the hindering of albumin denaturation. Overall, the ethyl acetate fraction was the richest in phlorotannins (9.4 +/- 0.03 mg PGE/g) and was also the most promising regarding the tested bioactive properties. Of note, its inhibitory potential towards alpha-amylase was about nine times that of the commercial drug acarbose and its inhibitory activity against high temperature-induced protein denaturation was superior to that of the non-steroidal drug ketoprofen. According to UHPLC-DAD-ESI-MS/MS analysis, this fraction contained a range of phlorotannins with at least six units of phloroglucinol, including dibenzodioxine-1,3,6,8-tetraol, fuhalol, pentaphlorethol, fucopentaphlorethol and dihydroxypentafuhalol, in addition to several less common phlorotannin sulfate derivatives.
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