期刊
ANTIOXIDANTS
卷 11, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/antiox11050981
关键词
antioxidant; anti-inflammatory; plant extract; reactive oxygen species (ROS); nuclear factor-kappa B (NF kappa B); inducible nitric oxide synthase (iNOS); cyclooxygenase-2 (COX-2); interleukin-6 (IL-6)
资金
- Council of Agriculture [105-107 AS-16.5.1MS-M1]
This study evaluated the antioxidant and anti-inflammatory activities of Ficus awkeotsang Makino extract (FAE) on fibroblasts and mouse models. The results showed that FAE exhibited strong antioxidant and anti-inflammatory effects, and could inhibit UVB-induced inflammation. These findings highlight the potential of FAE as a versatile adjuvant against free radical damage in pharmaceutical applications.
Antioxidant and anti-inflammatory activities of Ficus awkeotsang Makino extract (FAE) on Hs68 fibroblasts and BALB/c nude-mouse models are evaluated in this study. FAE was found to be non-toxic and showed high levels of DPPH, H2O2, and hydroxyl radical scavenging abilities; a ferrous chelating capacity; as well as ferric-reducing antioxidant capability. The antioxidant activity of FAE was strongly associated with polyphenolic content (flavonoids at 10.3 mg QE g(-1) and total phenol at 107.6 mg GAE g(-1)). The anti-inflammatory activity of FAE and the underlying molecular mechanisms were also investigated. The a* value of the mouse dorsal skin after treatment with FAE at 1.5 mg/mL in addition to chronic UVB exposure was found to decrease by 19.2% during a ten-week period. The anti-inflammatory effect of FAE was evidenced by the decreased accumulation of inflammatory cells and skin thickness. Expression levels of UVB-induced inflammatory proteins, including ROS, NF-kappa B, iNOS, COX-2, and IL-6, were significantly reduced upon FAE treatment in vitro and in vivo. Collectively, our results suggest that the inhibition of ROS and UVB-induced activation of the NF-kappa B downstream signaling pathway by FAE, indicating considerable potential as a versatile adjuvant against free radical damage in pharmaceutical applications.
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