期刊
BIOMOLECULES
卷 12, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/biom12030411
关键词
self-assembling peptides; RAD16-I; scaffold; degradation; proteolysis; beta-sheet; random coil
资金
- MIT, Cambridge, MA, USA
- IQS-School of Engineering, Barcelona, Spain [NIH 1-ROI-EB003805-01A1]
- LIVEBIOMAT (6th Frame Program, EU) [013653]
This study investigates the protease sensitivity of the self-assembling peptide RAD16-I, and demonstrates that it can be enzymatically degraded into short peptide sequences and further degraded into single amino acids, making it suitable for applications in tissue engineering and regenerative medicine.
One of the most desirable properties that biomaterials designed for tissue engineering or drug delivery applications should fulfill is biodegradation and resorption without toxicity. Therefore, there is an increasing interest in the development of biomaterials able to be enzymatically degraded once implanted at the injury site or once delivered to the target organ. In this paper, we demonstrate the protease sensitivity of self-assembling amphiphilic peptides, in particular, RAD16-I (AcN-RADARADARADARADA-CONH2), which contains four potential cleavage sites for trypsin. We detected that when subjected to thermal denaturation, the peptide secondary structure suffers a transition from beta-sheet to random coil. We also used Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF) to detect the proteolytic breakdown products of samples subjected to incubation with trypsin as well as atomic force microscopy (AFM) to visualize the effect of the degradation on the nanofiber scaffold. Interestingly, thermally treated samples had a higher extent of degradation than non-denatured samples, suggesting that the transition from beta-sheet to random coil leaves the cleavage sites accessible and susceptible to protease degradation. These results indicate that the self-assembling peptide can be reduced to short peptide sequences and, subsequently, degraded to single amino acids, constituting a group of naturally biodegradable materials optimal for their application in tissue engineering and regenerative medicine.
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