期刊
BIOMOLECULES
卷 12, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/biom12050715
关键词
diols; metabolic engineering; carbon sources; regulation; Escherichia coli
资金
- National Natural Science Foundation of China [31961133014]
- National Key R&D Program of China [2021YFC2100500]
- Fundamental Research Funds for the Central Universities
This article comprehensively summarizes the biosynthetic pathways of diols from renewable biomass in Escherichia coli and discusses the metabolic-engineering strategies and dynamic regulation methods to enhance diol production. The challenges in diol biosynthesis and potential methods to improve the diol-producing ability of the host are also proposed.
As bulk chemicals, diols have wide applications in many fields, such as clothing, biofuels, food, surfactant and cosmetics. The traditional chemical synthesis of diols consumes numerous non-renewable energy resources and leads to environmental pollution. Green biosynthesis has emerged as an alternative method to produce diols. Escherichia coli as an ideal microbial factory has been engineered to biosynthesize diols from carbon sources. Here, we comprehensively summarized the biosynthetic pathways of diols from renewable biomass in E. coli and discussed the metabolic-engineering strategies that could enhance the production of diols, including the optimization of biosynthetic pathways, improvement of cofactor supplementation, and reprogramming of the metabolic network. We then investigated the dynamic regulation by multiple control modules to balance the growth and production, so as to direct carbon sources for diol production. Finally, we proposed the challenges in the diol-biosynthesis process and suggested some potential methods to improve the diol-producing ability of the host.
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