4.5 Article

Enabling genomic island prediction and comparison in multiple genomes to investigate bacterial evolution and outbreaks

期刊

MICROBIAL GENOMICS
卷 8, 期 5, 页码 -

出版社

MICROBIOLOGY SOC
DOI: 10.1099/mgen.0.000818

关键词

antimicrobial resistance; comparative genomics; genomic islands; interactive visualization; web server

资金

  1. Swiss National Science Foundation [P300-PA_164673]
  2. Societe Academique Vaudoise
  3. Genome Canada
  4. Canadian Institutes of Health Research (CIHR)
  5. NSERC-CREATE (Natural Sciences and Engineering Research Council of Canada)
  6. CIHR
  7. Simon Fraser University
  8. Cisco Research Chair in Bioinformatics
  9. David Braley Chair in Computational Biology

向作者/读者索取更多资源

Outbreaks of virulent and/or drug-resistant bacteria have a significant impact on human health and major economic consequences. Genomic islands (GIs) are of high interest due to their encoding of virulence factors, antimicrobial-resistance (AMR) genes, and other adaptations. However, current computational methods for GI analysis are not suitable for rapid and accurate comparative analysis. IslandCompare is an open-source computational pipeline that enables GI prediction and comparison across multiple bacterial genomes. It provides a dynamic visualization of bacterial core-genome phylogeny and overlays GI predictions and AMR determinants. IslandCompare is accessible through a web-based interface, making it user-friendly for bioinformaticians, biologists, and clinicians.
Outbreaks of virulent and/or drug-resistant bacteria have a significant impact on human health and major economic consequences. Genomic islands (GIs; defined as clusters of genes of probable horizontal origin) are of high interest because they disproportionately encode virulence factors, some antimicrobial-resistance (AMR) genes, and other adaptations of medical or environmental interest. While microbial genome sequencing has become rapid and inexpensive, current computational methods for GI analysis are not amenable for rapid, accurate, user-friendly and scalable comparative analysis of sets of related genomes. To help fill this gap, we have developed IslandCompare, an open-source computational pipeline for GI prediction and comparison across several to hundreds of bacterial genomes. A dynamic and interactive visualization strategy displays a bacterial core-genome phylogeny, with bacterial genomes linearly displayed at the phylogenetic tree leaves. Genomes are overlaid with GI predictions and AMR determinants from the Comprehensive Antibiotic Resistance Database (CARD), and regions of similarity between the genomes are also displayed. GI predictions are performed using Sigi-HMM and IslandPath-DIMOB, the two most precise GI prediction tools based on nucleotide composition biases, as well as a novel blast-based consistency step to improve cross-genome prediction consistency. GIs across genomes sharing sequence similarity are grouped into clusters, further aiding comparative analysis and visualization of acquisition and loss of mobile GIs in specific sub-clades. IslandCompare is an open-source software that is containerized for local use, plus available via a user-friendly, web-based interface to allow direct use by bioinformaticians, biologists and clinicians (at https://islandcompare.ca).

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