4.7 Article

FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.871933

关键词

insect cells; recombinant proteins; S2 cells; glycoproteins; secreted proteins; structural biology

向作者/读者索取更多资源

Recombinant protein expression in eukaryotic insect cells, particularly using Drosophila melanogaster S2 cells, has great potential for challenging targets. The development of FAS2FURIOUS provides a simple and rapid platform for cloning and testing multiple constructs in a short period, making it an attractive orthogonal approach for protein expression in eukaryotic cells.
Recombinant protein expression in eukaryotic insect cells is a powerful approach for producing challenging targets. However, due to incompatibility with standard baculoviral platforms and existing low-throughput methodology, the use of the Drosophila melanogaster S2 cell line lags behind more common insect cell lines such as Sf9 or High-Five (TM). Due to the advantages of S2 cells, particularly for secreted and secretable proteins, the lack of a simple and parallelizable S2-based platform represents a bottleneck, particularly for biochemical and biophysical laboratories. Therefore, we developed FAS2FURIOUS, a simple and rapid S2 expression pipeline built upon an existing low-throughput commercial platform. FAS2FURIOUS is comparable in effort to simple E. coli systems and allows users to clone and test up to 46 constructs in just 2 weeks. Given the ability of S2 cells to express challenging targets, including receptor ectodomains, secreted glycoproteins, and viral antigens, FAS2FURIOUS represents an attractive orthogonal approach for protein expression in eukaryotic cells.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据