期刊
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2022.891538
关键词
blood endothelial cells; lymphatic endothelial cells; epigenetics; ATAC-seq; ChIP-seq; enhancers; conserved non-coding elements; chromatin structure
资金
- Wallenberg Academy Fellowship [2017.0144]
- Vetenskapsadet [VR-MH-2016-01437]
- Jeanssons Foundation
- Birmingham Fellowship (University of Birmingham, United Kingdom)
- Ragnar Soederbergs Fellowship [M13/17]
Epigenetic regulation plays a crucial role in the spatiotemporal regulation of gene expression, particularly in the field of vascular biology in zebrafish. The latest technologies enable researchers to assess genome-wide epigenetic modifications and validate the activity of cis-regulatory elements in zebrafish models. This research has led to breakthroughs in identifying and characterizing key enhancers of important vascular genes.
Epigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish represents a powerful model to investigate the activity of cis-regulatory elements in vivo by combining technologies such as ATAC-seq, ChIP-seq and CUT&Tag with the generation of transgenic lines and live imaging to validate the activity of these regulatory elements. Recently, this approach led to the identification and characterization of key enhancers of important vascular genes, such as gata2a, notch1b and dll4. In this review we will discuss how the latest technologies in epigenetics are being used in the zebrafish to determine chromatin states and assess the function of the cis-regulatory sequences that shape the zebrafish vascular network.
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