Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis

Mammalian spermatogenesis is associated with the transient appearance of condensed mitochondria, a singularity of germ cells with unknown function. Using proteomic analysis, respirometry, and electron microscopy with tomography, we studied the development of condensed mitochondria. Condensed mitochondria arose from orthodox mitochondria during meiosis by progressive contraction of the matrix space, which was accompanied by an initial expansion and a subsequent reduction of the surface area of the inner membrane. Compared to orthodox mitochondria, condensed mitochondria respired more actively, had a higher concentration of respiratory enzymes and supercomplexes, and contained more proteins involved in protein import and expression. After the completion of meiosis, the abundance of condensed mitochondria declined, which coincided with the onset of the biogenesis of acrosomes. Immuno-electron microscopy and the analysis of sub-cellular fractions suggested that condensed mitochondria or their fragments were translocated into the lumen of the acrosome. Thus, it seems condensed mitochondria are formed from orthodox mitochondria by extensive transformations in order to support the formation of the acrosomal matrix.

Automated summary

Mammalian spermatogenesis involves the transformation of conventional mitochondria into condensed mitochondria, which exhibit higher respiratory activity, enzyme concentration, and protein expression related to protein import. The abundance of condensed mitochondria decreases after their formation, coinciding with the onset of acrosome biogenesis. It is suggested that condensed mitochondria or their fragments are translocated into the lumen of the acrosome to support the formation of the acrosomal matrix.