期刊
REGENERATIVE THERAPY
卷 20, 期 -, 页码 107-116出版社
ELSEVIER
DOI: 10.1016/j.reth.2022.03.007
关键词
Lung cancer; Human bone marrow mesenchymal stem; cell; Exosomes; MicroRNA-425; Cytoplasmic polyadenylation binding; protein 1; Epithelial-mesenchymal transition; Migration; Invasion; Metastasis
资金
- Special project of Respiratory Clinical Medicine Research Center [201912011]
This study found that BMSC-Exos could inhibit CPEB1 expression in lung cancer cells by delivering miR-425, promoting the malignant biological properties of lung cancer cells and their metastasis.
Objective: Bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) could mediate the malignancy of tumor cells by transmitting targeted cargo. Therein, this study intends to explore the function of BMSC-Exos transmitting microRNA-425 (miR-425)/cytoplasmic polyadenylation binding protein 1 (CPEB1) in lung cancer growth. Methods: miR-425 and CPEB1 levels in cancer tissues and cells were measured. BMSCs and their exosomes were collected and identified. After intervention with BMSC-Exos, miR-425 or CPEB1, invasion and migration of A549 and NCI-H1299 cells in vitro, and lung metastasis of A549 cells in vivo were observed. The relationship between miR-425 and CPEB1 was verified. Results: miR-425 was highly expressed while CPEB1 was lowly expressed in lung cancer tissues of patients. CPEB1 was the direct target of miR-425. Down-regulating miR-425 or up-regulating CPEB1 decreased cell invasion and migration ability of A549 and NCI-H1299 cells, as well as decreased the number of lung metastasis lesions in vivo. After co-culture with BMSC-Exos, A549 and NCI-H1299 cells showed promoted migration and invasion in vitro and A549 cells demonstrated increased lung metastasis in vivo. Down-regulated miR-425 or up-regulated CPEB1 reversed the promotion of BMSC-Exos on lung cancer cell invasion, migration and lung metastasis. Conclusion: BMSC-Exos could deliver miR-425 to inhibit CPEB1 expression in lung cancer cells, thereby promoting the malignant biological properties of lung cancer cells and their metastasis in vivo. ?? 2022, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
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