Serine Hydroxymethyltransferase (SHMT) Gene Family in Wheat (Triticum aestivum L.): Identification, Evolution, and Expression Analysis

Serine hydroxymethyltransferase (SHMT) plays a vital role in one-carbon metabolic, photorespiration, and various stress responses. However, the genome-wide analysis has not been performed in wheat. In this study, a total of 12 TaSHMT genes were identified in wheat and classified into groups Ia, Ib, and IIb. TaSHMT genes in each group shared similar conserved domain distributions. Chromosomal location, synteny, and cis-elements analysis of TaSHMTs were also analyzed. Real-time PCR results indicated that most TaSHMT genes were mainly expressed in leaves and stems during the wheat seedling stage. Most TaSHMT genes could respond to various abiotic stress. The growth of yeast cells expressing TaSHMT2.1 was inhibited under salt and dehydration stress. Moreover, the gene ontology (GO) annotation and protein interaction of TaSHMT genes were analyzed. These results increase our understanding of SHMT genes and provide robust candidate genes for further functional investigations aimed at crop improvement.

Automated summary

In this study, a genome-wide analysis of serine hydroxymethyltransferase (SHMT) in wheat was performed, identifying 12 TaSHMT genes classified into different groups. The results showed that these genes were mainly expressed in leaves and stems during the seedling stage and could respond to various abiotic stress. The chromosomal location, synteny, and cis-elements analysis of TaSHMTs were also investigated, along with gene ontology annotation and protein interaction analysis.