期刊
FOOD SCIENCE & NUTRITION
卷 10, 期 7, 页码 2470-2475出版社
WILEY
DOI: 10.1002/fsn3.2853
关键词
FecB; PCR-RFLP; SNPs; TaqMan
资金
- Xilingol Science and Technology Planning Project [202103]
- Inner Mongolia College Science Research Project [NJZY22711]
- Xilingol Vocational College Key Research Project [ZD-2021-01, YB-2022-13]
This study investigated SNPs in the FecB locus of Xilingol indigenous sheep breeds using novel techniques, laying the groundwork for future authentication and breeding strategies.
The muscle from Xilingol indigenous sheep breeds are famous in China, and the FecB genotype in this population remains uncharacterized. In this study, SNPs in the FecB locus were investigated by pyrosequencing, and an optimized PCR-RFLP technique was generated to identify SNPs. In addition, an efficient technique for high-throughput identification of SNPs in FecB was optimized using TaqMan real-time PCR and breed-conservative primers and SNP-specific probes. By genotyping the FecB locus in the muscle of Xilingol indigenous sheep breeds using a novel TaqMan real-time PCR assay, our study has generated the groundwork for the authentication of Xilingol mutton based on the specific gene and the prolificacy-oriented breeding of Xilingol sheep using marker-assisted selection strategies in the future.
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