Effect of Fish Stock Density on Hormone Genes Expression from Brain and Gastrointestinal Tract of Salmo salar

Simple Summary Various long-term stress conditions may exist in fish cultivation, damaging the physiological responses that regulate the fish growth and feed. Different signalers connect the brain with the gastrointestinal tract, including the perception of stress factors for the regulation of physiological responses. Here, we evaluated the effect of varying culture densities of Salmo salar post-smolt on the gene expression of some brain and gastrointestinal hormone signalers. We found that high stock densities could promote the levels of molecules associated with feed inhibition, which could be related to the stress pathway regulated by corticoids. Thus, the expression of these peptide hormones could be used as biological markers to improve production practices in fish aquaculture. A variety of long-term stress conditions may exist in fish cultivation, some of which are so severe that fish can no longer reestablish homeostasis. In teleost fish, the brain and gastrointestinal tract integrate signals that include the perception of stress factors regulating physiological responses, such as social stress by fish population density, where peripheral and central signals, such as peptide hormones, are the main regulators. Therefore, we proposed in this study to analyze the effect of different stock densities (SD) in the gene expression of brain neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP), together with the gastrointestinal peptide hormones leptin (Lep), vasointestinal peptide (VIP), and protachykinin-1 (Prk-1) in Salmo salar post-smolt. The coding sequence of S. salar VIP and Prk-1 precursors were firstly cloned and characterized. Then, the mRNA expression of these genes, together with the NPY, Lep, and CGRP genes, were evaluated in post-smolts kept at 11 Kg/m(3), 20 Kg/m(3), and 40 Kg/m(3). At 14 days of culture, the brain CGRP and liver leptin mRNA levels increased three and tenfold in the post-smolt salmons kept at the highest SD, respectively. The high levels of leptin were kept during all the fish culture experiments. In addition, the highest expression of intestine VIP mRNA was obtained on Day 21 in the group of 40 Kg/m(3) returning to baseline on Day 40. In terms of stress biochemical parameters, cortisol levels were increased in the 20 Kg/m(3) and 40 Kg/m(3) groups on Day 40 and were the highest in the 20 Kg/m(3) group on Day 14. This study provides new insight into the gastrointestinal signals that could be affected by chronic stress induced by high stock density in fish farming. Thus, the expression of these peptide hormones could be used as molecular markers to improve production practices in fish aquaculture.

Automated summary

This study evaluated the effect of different stock densities on the gene expression of brain and gastrointestinal hormone signalers in Salmo salar post-smolt. High stock densities were found to promote the levels of molecules associated with feed inhibition, which could be used as biological markers to improve production practices in fish aquaculture.