Expression of Oocyte Vitellogenesis Receptor Was Regulated by C/EBPα in Developing Follicle of Wanxi White Goose

Yolk precursor was synthesized under regulation of hormone secretion, while the mechanism of its incorporation into follicle is still unknown. The reproductive hormones, oocyte vitellogenesis receptor (OVR) expression at pre-, early-, peak- and ceased-laying period, and localization of Wanxi White goose were determined in this study. The results showed that the concentration of LH was lowest in serum at peak laying period compared to the other periods (p < 0.01). Moreover, the concentration of E2 was highest (p < 0.01) in serum at early laying period than that of other periods. Moreover, the gene expression level of OVR was highest at ceased laying period compared to other periods (p = 0.014) and was higher in developing follicles than other follicles (p < 0.01). The OVR was distributed in the granular cell layer and decreased with the maturation of follicles. Five transcription factors were predicted in the promoter of OVR, then were screened and verified by overexpression in granulosa cells. C/EBP alpha and MF3 significantly stimulated the expression of OVR. The combined overexpression of C/EBP alpha and OVR significantly stimulated the transportation of lipid from culture medium to cytoplasm. In conclusion, C/EBP alpha is the key transcription factor promoting OVR expression in goose follicle granulosa cells.

Automated summary

This study investigated the reproductive hormones, expressions of oocyte vitellogenesis receptor (OVR) at different laying periods, and localization in Wanxi White goose. The results showed that LH concentration was lowest during peak laying period, while E2 concentration was highest during early laying period. The gene expression of OVR was highest at ceased laying period and higher in developing follicles. C/EBP alpha was identified as the key transcription factor promoting OVR expression in goose follicle granulosa cells.