Identification of Genes Related to Squab Muscle Growth and Lipid Metabolism from Transcriptome Profiles of Breast Muscle and Liver in Domestic Pigeon (Columba livia)

Simple Summary Domestic pigeon is an important small poultry species raised for high-quality meat production. However, the relevant gene associated with meat growth and lipid metabolism during the period from dehulling to marketing are not known. Therefore, we aim to identify genes related to squab muscle growth and lip metabolism from transcriptome profiles of breast muscle and liver in domestic pigeon. In this study, we totally found that 4465 differentially expressed genes (DEGs) identified in the breast muscle and liver libraries, which include 2585 genes that were up-regulated and 2122 genes that were down-regulated. Most genes are involved in cell proliferation and differentiation, lipid metabolism and energy metabolism according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs. We also detected 16 DEGs to verify data from RNA-Seq and qPCR, which were consistent in quantitatively estimating the transcription levels of the tested transcripts by qPCR analysis. The results of this study will lay the foundation for understanding the mechanisms of muscle growth and lipid metabolism in domestic pigeons. The improvements in muscle growth rate and meat quality are the major breeding aims in pigeon industry. Liver and muscle are recognized as important sites for fatty acid metabolism; understanding the role of specific transcripts in the breast muscle and liver might lead to the elucidation of interrelated biological processes. In this study, RNA-Sequencing (RNA-Seq) was applied to compare the transcriptomes of breast muscle and liver tissues among pigeons at five developmental periods (0, 1, 2, 3, 4 weeks post-hatching) to identify candidate genes related to muscle growth and lipid metabolism. There were 3142 differentially expressed genes (DEGs) identified in the breast muscle libraries; 1794 genes were up-regulated while 1531 genes were down-regulated. A total of 1323 DEGs were acquired from the liver libraries, with 791 up-regulated genes and 591 down-regulated genes. By pathway enrichment analysis, a set of significantly enriched pathways were identified for the DEGs, which are potentially involved in cell proliferation and differentiation, lipid metabolism and energy metabolism in pigeon breast muscle and liver. Our results are consistent with previous partial reports from domestic animals and poultry and provide some unidentified genes involved in muscle growth and lipid metabolism. The reliability of the sequencing data was verified through qPCR analysis of 16 genes from eight comparison groups (two genes per group). The findings from this study could contribute to future investigations of muscle growth and lipid metabolism mechanisms and establish molecular approaches to improve muscle growth rate and meat quality in domestic pigeon breeding.

Automated summary

This study identified differentially expressed genes related to muscle growth and lipid metabolism in domestic pigeons by analyzing the transcriptomes of breast muscle and liver. The identified genes are involved in cell proliferation and differentiation, lipid metabolism, and energy metabolism. The findings lay the foundation for understanding the mechanisms of muscle growth and lipid metabolism in domestic pigeons.