4.7 Article

PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing in Mycobacteria

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biotechnology & Applied Microbiology

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

Ibrahim C. Kurt et al.

Summary: This study presents two base editor architectures that can efficiently induce targeted C-to-G base transversions, with reduced levels of unwanted mutations. These new base editors hold promise for optimizing C-to-G base editors for both research and therapeutic applications.

NATURE BIOTECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

Glycosylase base editors enable C-to-A and C-to-G base changes

Dongdong Zhao et al.

Summary: The study introduces novel glycosylase base editors (GBEs) that can induce C-to-A and C-to-G transversions in Escherichia coli and mammalian cells, respectively. The new editors exhibit high editing specificity and efficiency, making them potential tools for targeting G/C disease-causing mutations.

NATURE BIOTECHNOLOGY (2021)

Article Multidisciplinary Sciences

Programmable C:G to G:C genome editing with CRISPR-Cas9-directed base excision repair proteins

Liwei Chen et al.

Summary: A new class of base editors, C:G to G:C Base Editors, have been developed in this study to create single-base genomic transversions in human cells, targeting specific genes related to genetic diseases. These editors predominantly perform C:G to G:C editing with high purity and efficiency, showing therapeutic potential for interrogating and correcting human genetic diseases.

NATURE COMMUNICATIONS (2021)

Article Biochemistry & Molecular Biology

A Cas12a-based CRISPR interference system for multigene regulation in mycobacteria

Neil Fleck et al.

Summary: An alternative CRISPRi platform based on the minimal type V Cas12a enzyme in combination with synthetic CRISPR arrays is introduced for mycobacteria. This system is simple, tunable, reversible, and efficiently regulates essential genes and multiple genes simultaneously, working as effectively in infected macrophages as it does in vitro. Cas12a-based CRISPRi provides a facile tool to investigate higher-order genetic interactions in mycobacteria, including Mycobacterium tuberculosis (Mtb), for the development of synthetically lethal drug targets and the study of conditionally essential genes during infection.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Article Biotechnology & Applied Microbiology

Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning

Luke W. Koblan et al.

Summary: Engineered CGBEs paired with machine learning models have been developed to enable efficient and high-purity base editing, correcting disease-related SNVs with precision and increasing editing efficiency. Matching a set of base editors to target sequences can further enhance transversion base editing efficiency.

NATURE BIOTECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

Glycosylase base editors enable C-to-A and C-to-G base changes

Dongdong Zhao et al.

Summary: This study presents new glycosylase base editors (GBEs) that can induce C-to-A and C-to-G transversions in bacteria and mammalian cells, respectively. The GBEs can serve as a complement to existing adenine and cytidine base editors (ABE and CBE) and have the potential to target G/C disease-causing mutations.

NATURE BIOTECHNOLOGY (2021)

Article Chemistry, Physical

Catalytic-state structure and engineering of Streptococcus thermophilus Cas9

Yifei Zhang et al.

NATURE CATALYSIS (2020)

Article Biotechnology & Applied Microbiology

CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii

Qi Li et al.

BIOTECHNOLOGY AND BIOENGINEERING (2019)

Article Multidisciplinary Sciences

Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST

Yaojun Tong et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Article Biotechnology & Applied Microbiology

MACBETH: Multiplex automated Corynebacterium glutamicum base editing method

Yu Wang et al.

METABOLIC ENGINEERING (2018)

Article Biotechnology & Applied Microbiology

Base editing with a Cpf1-cytidine deaminase fusion

Xiaosa Li et al.

NATURE BIOTECHNOLOGY (2018)

Article Microbiology

Deaminase-mediated multiplex genome editing in Escherichia coli

Satomi Banno et al.

NATURE MICROBIOLOGY (2018)

Article Biotechnology & Applied Microbiology

CRISPR-Cas9 and CRISPR-Assisted Cytidine Deaminase Enable Precise and Efficient Genome Editing in Klebsiella pneumoniae

Yu Wang et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2018)

Article Genetics & Heredity

EditR: A Method to Quantify Base Editing from Sanger Sequencing

Mitchell G. Kluesner et al.

CRISPR JOURNAL (2018)

Article Chemistry, Multidisciplinary

Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System

Weizhong Chen et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Article Multidisciplinary Sciences

Programmable base editing of A.T to G.C in genomic DNA without DNA cleavage

Nicole M. Gaudelli et al.

NATURE (2017)

Article Medicine, Research & Experimental

CRISPR/Cas9-loxP- Mediated Gene Editing as a Novel Site-Specific Genetic Manipulation Tool

Fayu Yang et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2017)

Article Biotechnology & Applied Microbiology

Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System

Josef Altenbuchner

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2016)

Review Infectious Diseases

Overview on mechanisms of isoniazid action and resistance in Mycobacterium tuberculosis

Ameeruddin Nusrath Unissa et al.

INFECTION GENETICS AND EVOLUTION (2016)

Article Multidisciplinary Sciences

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Alexis C. Komor et al.

NATURE (2016)

Article Biochemistry & Molecular Biology

Investigating essential gene function in Mycobacterium tuberculosis using an efficient CRISPR interference system

Atul K. Singh et al.

NUCLEIC ACIDS RESEARCH (2016)

Article Medicine, Research & Experimental

CRISPR/Cas9-AAV Mediated Knock-in at NRL Locus in Human Embryonic Stem Cells

Xianglian Ge et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2016)

Article Biotechnology & Applied Microbiology

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

Yu Jiang et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2015)

Article Multidisciplinary Sciences

Gene silencing by CRISPR interference in mycobacteria

Eira Choudhary et al.

NATURE COMMUNICATIONS (2015)

Article Biochemical Research Methods

CRISPR-Cas9 Based Engineering of Actinomycetal Genomes

Yaojun Tong et al.

ACS SYNTHETIC BIOLOGY (2015)

Article Biochemical Research Methods

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

Ryan E. Cobb et al.

ACS SYNTHETIC BIOLOGY (2015)

Article Biochemistry & Molecular Biology

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

Luke A. Gilbert et al.

Article Biotechnology & Applied Microbiology

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

Wenyan Jiang et al.

NATURE BIOTECHNOLOGY (2013)

Article Multidisciplinary Sciences

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali et al.

SCIENCE (2013)

Article Multidisciplinary Sciences

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong et al.

SCIENCE (2013)

Letter Immunology

Totally Drug-Resistant Tuberculosis in India

Zarir F. Udwadia et al.

CLINICAL INFECTIOUS DISEASES (2012)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Biochemical Research Methods

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson et al.

NATURE METHODS (2009)

Article Biochemical Research Methods

Recombineering in Mycobacterium tuberculosis

Julia C. van Kessel et al.

NATURE METHODS (2007)